rabbit anti pak5  (Proteintech)

Journal: Oncology Letters

Article Title: Correlation between chemotherapy resistance in osteosarcoma patients and PAK5 and Ezrin gene expression

doi: 10.3892/ol.2017.7355

Figure Lengend Snippet: PCR primer sequence of PAK5, Ezrin and GAPDH mRNA.

Article Snippet: Human osteosarcoma cell line SOSP-9607 (Shanghai Cell Bank, Chinese Academy of Sciences, Shanghai, China), methyl thiazolyltetrazolium (MTT) (Sigma; Merck KGaA, Darmstadt, Germany USA), dimethylsulfoxide (DMSO; Sigma; Merck KGaA), CDDP (Shanghai Aladdin Biochemical Technology Co., Ltd., Shanghai, China), TRIzol reagent (Invitrogen; Thermo Fisher Scientific, Inc., Waltham, MA, USA), reverse transcription kit (Invitrogen, Thermo Fisher Scientific, Inc.), ELISA kit (R&D Systems, Inc., Minneapolis, MN, USA), ECI luminescent solution (Invitrogen, Thermo Fisher Scientific, Inc.), rabbit anti-human PAK5 polyclonal primary antibody (dilution, 1:1,000; cat. no. 3241), goat anti-rabbit polyclonal secondary antibody (dilution, 1:500; cat. no. 7074) (both from Cell Signaling Technology, Inc., Boston, MA, USA), rabbit anti-Ezrin and glyceraldehyde 3-phosphate dehydrogenase (GAPDH; Cell Signaling Technology, Inc., Danvers, MA, USA), inversed fluorescent microscope (Thermo Electron LED GmbH, Langenselbold, Germany), cell culture flask (Corning Incorporated, Corning, NY, USA), pipettor (Eppendorf, Hamburg, Germany), Transwell chamber (Eppendorf), PCR instrument (ABI, Los Angeles, CA, USA), UV imaging system (Biometra GmbH, Göttingen, Germany), electronic balance (BP121S; Sartorius AG, Göttingen, Germany) were obtained; other related equipment and reagents are described in the relevant parts.

Techniques: Sequencing

Journal: Oncology Letters

Article Title: Correlation between chemotherapy resistance in osteosarcoma patients and PAK5 and Ezrin gene expression

doi: 10.3892/ol.2017.7355

Figure Lengend Snippet: Detection of PAK5 and Ezrin expression via semi-quantitative PCR. (A) Agarose gel electrophoresis chart. (B and C) Statistical charts of relative expression quantities of PAK5 and Ezrin; the results showed that PAK5 and Ezrin expression levels in SOSP-9607/CDDP was significantly higher than those in SOSP-9607 (**P<0.01).

Article Snippet: Human osteosarcoma cell line SOSP-9607 (Shanghai Cell Bank, Chinese Academy of Sciences, Shanghai, China), methyl thiazolyltetrazolium (MTT) (Sigma; Merck KGaA, Darmstadt, Germany USA), dimethylsulfoxide (DMSO; Sigma; Merck KGaA), CDDP (Shanghai Aladdin Biochemical Technology Co., Ltd., Shanghai, China), TRIzol reagent (Invitrogen; Thermo Fisher Scientific, Inc., Waltham, MA, USA), reverse transcription kit (Invitrogen, Thermo Fisher Scientific, Inc.), ELISA kit (R&D Systems, Inc., Minneapolis, MN, USA), ECI luminescent solution (Invitrogen, Thermo Fisher Scientific, Inc.), rabbit anti-human PAK5 polyclonal primary antibody (dilution, 1:1,000; cat. no. 3241), goat anti-rabbit polyclonal secondary antibody (dilution, 1:500; cat. no. 7074) (both from Cell Signaling Technology, Inc., Boston, MA, USA), rabbit anti-Ezrin and glyceraldehyde 3-phosphate dehydrogenase (GAPDH; Cell Signaling Technology, Inc., Danvers, MA, USA), inversed fluorescent microscope (Thermo Electron LED GmbH, Langenselbold, Germany), cell culture flask (Corning Incorporated, Corning, NY, USA), pipettor (Eppendorf, Hamburg, Germany), Transwell chamber (Eppendorf), PCR instrument (ABI, Los Angeles, CA, USA), UV imaging system (Biometra GmbH, Göttingen, Germany), electronic balance (BP121S; Sartorius AG, Göttingen, Germany) were obtained; other related equipment and reagents are described in the relevant parts.

Techniques: Expressing, Real-time Polymerase Chain Reaction, Agarose Gel Electrophoresis

Journal: Oncology Letters

Article Title: Correlation between chemotherapy resistance in osteosarcoma patients and PAK5 and Ezrin gene expression

doi: 10.3892/ol.2017.7355

Figure Lengend Snippet: Detection of PAK5 and Ezrin protein expression levels via western blot analysis. (A) Bar graph of western blot analysis. (B and C) Statistical charts of PAK5 and Ezrin protein expression quantities. The results showed that PAK5 and Ezrin protein expression levels in SOSP-9607/CDDP were significantly higher than those in SOSP-9607 (**P<0.01).

Article Snippet: Human osteosarcoma cell line SOSP-9607 (Shanghai Cell Bank, Chinese Academy of Sciences, Shanghai, China), methyl thiazolyltetrazolium (MTT) (Sigma; Merck KGaA, Darmstadt, Germany USA), dimethylsulfoxide (DMSO; Sigma; Merck KGaA), CDDP (Shanghai Aladdin Biochemical Technology Co., Ltd., Shanghai, China), TRIzol reagent (Invitrogen; Thermo Fisher Scientific, Inc., Waltham, MA, USA), reverse transcription kit (Invitrogen, Thermo Fisher Scientific, Inc.), ELISA kit (R&D Systems, Inc., Minneapolis, MN, USA), ECI luminescent solution (Invitrogen, Thermo Fisher Scientific, Inc.), rabbit anti-human PAK5 polyclonal primary antibody (dilution, 1:1,000; cat. no. 3241), goat anti-rabbit polyclonal secondary antibody (dilution, 1:500; cat. no. 7074) (both from Cell Signaling Technology, Inc., Boston, MA, USA), rabbit anti-Ezrin and glyceraldehyde 3-phosphate dehydrogenase (GAPDH; Cell Signaling Technology, Inc., Danvers, MA, USA), inversed fluorescent microscope (Thermo Electron LED GmbH, Langenselbold, Germany), cell culture flask (Corning Incorporated, Corning, NY, USA), pipettor (Eppendorf, Hamburg, Germany), Transwell chamber (Eppendorf), PCR instrument (ABI, Los Angeles, CA, USA), UV imaging system (Biometra GmbH, Göttingen, Germany), electronic balance (BP121S; Sartorius AG, Göttingen, Germany) were obtained; other related equipment and reagents are described in the relevant parts.

Techniques: Expressing, Western Blot

Journal: Oncology Letters

Article Title: Correlation between chemotherapy resistance in osteosarcoma patients and PAK5 and Ezrin gene expression

doi: 10.3892/ol.2017.7355

Figure Lengend Snippet: Detection of PAK5 expression via immunohistochemistry. (A) PAK5 expression level in osteosarcoma. (B) PAK5 expression level in para-tumor tissue; the results showed that PAK5 positive expression rate in osteosarcoma was significantly higher than that in para-tumor tissue.

Article Snippet: Human osteosarcoma cell line SOSP-9607 (Shanghai Cell Bank, Chinese Academy of Sciences, Shanghai, China), methyl thiazolyltetrazolium (MTT) (Sigma; Merck KGaA, Darmstadt, Germany USA), dimethylsulfoxide (DMSO; Sigma; Merck KGaA), CDDP (Shanghai Aladdin Biochemical Technology Co., Ltd., Shanghai, China), TRIzol reagent (Invitrogen; Thermo Fisher Scientific, Inc., Waltham, MA, USA), reverse transcription kit (Invitrogen, Thermo Fisher Scientific, Inc.), ELISA kit (R&D Systems, Inc., Minneapolis, MN, USA), ECI luminescent solution (Invitrogen, Thermo Fisher Scientific, Inc.), rabbit anti-human PAK5 polyclonal primary antibody (dilution, 1:1,000; cat. no. 3241), goat anti-rabbit polyclonal secondary antibody (dilution, 1:500; cat. no. 7074) (both from Cell Signaling Technology, Inc., Boston, MA, USA), rabbit anti-Ezrin and glyceraldehyde 3-phosphate dehydrogenase (GAPDH; Cell Signaling Technology, Inc., Danvers, MA, USA), inversed fluorescent microscope (Thermo Electron LED GmbH, Langenselbold, Germany), cell culture flask (Corning Incorporated, Corning, NY, USA), pipettor (Eppendorf, Hamburg, Germany), Transwell chamber (Eppendorf), PCR instrument (ABI, Los Angeles, CA, USA), UV imaging system (Biometra GmbH, Göttingen, Germany), electronic balance (BP121S; Sartorius AG, Göttingen, Germany) were obtained; other related equipment and reagents are described in the relevant parts.

Techniques: Expressing, Immunohistochemistry

Journal: Oncology Letters

Article Title: Correlation between chemotherapy resistance in osteosarcoma patients and PAK5 and Ezrin gene expression

doi: 10.3892/ol.2017.7355

Figure Lengend Snippet: Correlation between PAK5 and Ezrin expression in osteosarcoma tissues.

Article Snippet: Human osteosarcoma cell line SOSP-9607 (Shanghai Cell Bank, Chinese Academy of Sciences, Shanghai, China), methyl thiazolyltetrazolium (MTT) (Sigma; Merck KGaA, Darmstadt, Germany USA), dimethylsulfoxide (DMSO; Sigma; Merck KGaA), CDDP (Shanghai Aladdin Biochemical Technology Co., Ltd., Shanghai, China), TRIzol reagent (Invitrogen; Thermo Fisher Scientific, Inc., Waltham, MA, USA), reverse transcription kit (Invitrogen, Thermo Fisher Scientific, Inc.), ELISA kit (R&D Systems, Inc., Minneapolis, MN, USA), ECI luminescent solution (Invitrogen, Thermo Fisher Scientific, Inc.), rabbit anti-human PAK5 polyclonal primary antibody (dilution, 1:1,000; cat. no. 3241), goat anti-rabbit polyclonal secondary antibody (dilution, 1:500; cat. no. 7074) (both from Cell Signaling Technology, Inc., Boston, MA, USA), rabbit anti-Ezrin and glyceraldehyde 3-phosphate dehydrogenase (GAPDH; Cell Signaling Technology, Inc., Danvers, MA, USA), inversed fluorescent microscope (Thermo Electron LED GmbH, Langenselbold, Germany), cell culture flask (Corning Incorporated, Corning, NY, USA), pipettor (Eppendorf, Hamburg, Germany), Transwell chamber (Eppendorf), PCR instrument (ABI, Los Angeles, CA, USA), UV imaging system (Biometra GmbH, Göttingen, Germany), electronic balance (BP121S; Sartorius AG, Göttingen, Germany) were obtained; other related equipment and reagents are described in the relevant parts.

Techniques: Expressing